Research Progress on bioactive peptides from animal sources: A comprehensive review

Bioactive peptides derived from animals have antitumor, antibacterial, antioxidant and other beneficial activities for human health. Successful application in preclinical models demonstrates the potential of animal-derived bioactive peptides to be developed into health foods and functional foods for the treatment of disease. The authors' prior studies have extensively investigated bioactive peptides, establishing a robust preparation and purification system. This review highlights optimized enzymatic hydrolysis methods that enhance peptide yield and bioactivity, combination separation methods that increase the efficiency of separating and purifying bioactive peptides, mass spectrometry methods that determine the molecular weight of bioactive peptides, their mechanisms of action and structure-activity relationships as well as the metabolic process of bioactive peptides in vivo.