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Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection
Stephanie Beane1, Megan Burch1,2, Jeremy Bechelli3,4
1Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2025
Summary
Colorado tick fever virus (CTFV) research is advanced by new protocols for viral quantification, stock generation, and detection. These methods address the underreporting of this significant tick-borne illness.
Area of Science:
- Virology
- Tick-borne diseases
- Reoviridae family
Background:
- Colorado tick fever virus (CTFV) causes Colorado tick fever (CTF), a potentially severe febrile illness.
- CTFV is underreported and underrecognized, with a lack of standardized research protocols for neglected tick-borne viruses.
Purpose of the Study:
- To establish standardized protocols for Colorado tick fever virus (CTFV) research.
- To facilitate further investigation into this underrecognized tick-borne virus.
Main Methods:
- Viral plaque assay for CTFV quantification.
- Procedures for the generation and storage of CTFV stocks.
- Reverse transcription-quantitative PCR (RT-qPCR) for CTFV detection.
Main Results:
- Established protocols for viral plaque assays, stock preparation, and RT-qPCR detection of CTFV.
- Provided a framework for consistent and reproducible CTFV research.
Conclusions:
- The developed protocols offer essential tools for advancing CTFV research.
- Standardized methods are crucial for studying underrecognized tick-borne viruses like CTFV.
Keywords:
Cell cultureColorado tick feverColorado tick fever virusTick-borne diseaseVero cellsViral detectionViral propagationViral quantificationMore Related Videos
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