Related Experiment Video
Updated: Apr 14, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
Spore display innovation technology for unlocking new substrates for glucose oxidase
Xihua Chen1, Zeyang Li1, Zhengfen Wu1
1School of Life Sciences, Jiangsu University, Zhenjiang, China.
Background:
Glucose oxidase (GOD) has been extensively applied in the animal feed industry. However, its substrate specificity limits its utility in the broad degradation of fibrous raw materials.
Results:
In this study, a series of spore display technologies were employed to transform GOD from an enzyme specific for β-d-glucose into a broad-spectrum sugar oxidase capable of catalyzing various sugars such as arabinose, xylose, sucrose, cellobiose, raffinose, and sorbitol. Notably, the relative enzyme activities for arabinose, xylose, and sorbitol increased from less than 5% to 71%, 52%, and 53%, respectively. Furthermore, the optimal temperature for GOD activity shifted from 30 °C to 60 °C, significantly improving its thermal stability.
Conclusion:
Enzymes with high activity, strong heat resistance, and the ability to catalyze various sugars can help reduce the accumulation of hydrolysis products in animal feed, improving the cost-effectiveness of the feed. This contributes to the advancement of sustainable agriculture and the further development of feed production. © 2025 Society of Chemical Industry.
Related Concept Videos
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
Outcomes of Glycolysis
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Glucose Absorption Into the Small Intestine
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are...

