Related Experiment Video
Updated: May 8, 2026
11:26
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
Published on: August 14, 2014
19.7K
Leukocyte transendothelial migration hotspots at a glance
Max L B Grönloh1, Werner J van der Meer1, Merel E Tebbens1
1Department of Medical Biochemistry, Amsterdam UMC, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands.
Journal of Cell Science
|June 17, 2025
Summary
Immune cells migrate through blood vessel walls at specific
Area of Science:
- Immunology
- Cell Biology
- Vascular Biology
Background:
- Leukocyte extravasation involves crossing endothelial and pericyte barriers.
- This process, including transendothelial migration (TEM) and trans-pericyte migration (TPM), occurs at specialized 'hotspots'.
Purpose of the Study:
- To overview mechanisms defining leukocyte extravasation hotspots.
- To highlight leukocyte-induced hotspot formation.
- To explore chemokine regulation and endothelial cell strategies during transmigration.
Main Methods:
- Review of existing literature and research findings.
- Synthesis of data on cellular heterogeneity and molecular mechanisms.
- Analysis of immune cell interactions with vascular and perivascular cells.
Main Results:
- Inflamed endothelial and perivascular cell heterogeneity defines extravasation hotspots.
- Leukocytes actively induce new hotspots during extravasation.
- Diverse mechanisms regulate chemokine presentation and endothelial barrier integrity.
Conclusions:
- Leukocyte extravasation is a regulated, hotspot-confined process.
- Understanding these mechanisms is crucial for immune cell trafficking and vascular integrity.
- This review provides a comprehensive perspective on leukocyte-vascular cell interactions.
