Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

756.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
756.9K
DNA Isolation01:24

DNA Isolation

40.1K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
40.1K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

54.1K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
54.1K
Next-generation Sequencing03:00

Next-generation Sequencing

92.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.5K
Proofreading01:31

Proofreading

6.6K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
6.6K
PCR01:32

PCR

226.4K
Overview
226.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative analysis of complete chloroplast genomes provides insights into phylogenetic relationships and screening of polymorphic markers in Dimocarpus and related species.

BMC plant biology·2026
Same author

PCN-224-Pt nanozyme for dual-mode detection of acetylcholinesterase <i>via</i>LMB-PEG.

Chemical communications (Cambridge, England)·2026
Same author

From Laboratory to Clinic: Translational Medicine Paradigm of Polymyxin B Nanopreparations for Overcoming Drug-Resistant Bacterial Infections.

International journal of nanomedicine·2026
Same author

Dense crystalline-amorphous heterointerface catalysts for freshwater/seawater splitting and small molecule synergistic electrolysis.

Chemical communications (Cambridge, England)·2026
Same author

Ultrasensitive Portable LAMP-Powered Microfluidic Device for On-Site Rapid Forensic Species and Sex Identification.

Analytical chemistry·2026
Same author

A Pt@MOF-derived branch-leaf nanoarray: a mechanically flexible and high-performance biosensor for non-enzymatic electrochemical glucose detection.

Analytical methods : advancing methods and applications·2026

Related Experiment Video

Updated: Sep 8, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.2K

Research progress in high-throughput DNA synthesis and its applications.

Chongyu Liu1, Weihua Zhuang1, Liheng Liu1

  • 1Department of Laboratory Medicine, Precision Medicine Translational Research Center, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. huwenchuang@wchscu.cn.

Journal of Materials Chemistry. B
|June 18, 2025
PubMed
Summary

High-throughput DNA synthesis technologies are advancing genomics and synthetic biology. Third-generation enzymatic methods offer sustainable, long-chain DNA synthesis, overcoming limitations of traditional and second-generation approaches.

More Related Videos

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

2.8K
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

14.2K

Related Experiment Videos

Last Updated: Sep 8, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.2K
Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

2.8K
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

14.2K

Area of Science:

  • Genomics and Synthetic Biology
  • Biotechnology
  • Molecular Biology

Background:

  • Traditional DNA synthesis methods (e.g., first-generation synthesizers, PCR) have limitations in synthesizing long DNA chains and large-scale parallel synthesis.
  • Second-generation high-throughput technologies (photolithographic, inkjet, electrochemical, thermal microarray synthesis) improve efficiency and throughput but face challenges with sequence length and environmental impact.
  • Third-generation enzymatic synthesis offers environmental sustainability and superior long-chain DNA synthesis capabilities.

Purpose of the Study:

  • To review and categorize mainstream high-throughput DNA synthesis technologies.
  • To analyze the advantages and limitations of traditional, second-generation, and third-generation synthesis methods.
  • To explore the applications and future potential of advanced DNA synthesis technologies in life sciences and medicine.

Main Methods:

  • Systematic review of high-throughput DNA synthesis technologies.
  • Categorization of synthesis methods based on technological generations and microarray integration.
  • Comparative analysis of efficiency, cost, throughput, sequence length, and environmental impact.

Main Results:

  • First-generation methods are limited for long DNA and parallel synthesis.
  • Second-generation methods offer improvements but have sequence length and environmental constraints.
  • Third-generation enzymatic synthesis shows promise for sustainable, long-chain DNA production.

Conclusions:

  • High-throughput DNA synthesis technologies are evolving, with enzymatic methods representing a significant advancement.
  • Continued innovation is crucial for overcoming current limitations and expanding applications in various scientific fields.
  • Future research should focus on enhancing sequence length, scalability, and environmental sustainability in DNA synthesis.