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Advancing Tumor Budding Detection With Fourier Ptychography Microscopy.

Yiyan Su1, Ruiqing Sun2, Yajing Wang3

  • 1Department of Health Statistics, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province, China.

Cancer Medicine
|June 18, 2025
PubMed
Summary

Fourier ptychographic microscopy (FPM) simplifies colorectal cancer diagnosis by enabling digital refocusing and marker removal. This advanced imaging technique achieved 100% diagnostic accuracy, streamlining pathology workflows.

Keywords:
colorectal cancercountfourier ptychographic microscopytumor budding

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Area of Science:

  • Digital pathology
  • Computational imaging
  • Optical microscopy

Background:

  • Tumor budding is a critical prognostic indicator in colorectal cancer, necessitating detailed pathological examination.
  • Conventional microscopy for tumor budding assessment involves time-consuming manual adjustments and objective switching.
  • Existing methods require manual removal of slide markers, adding complexity to the diagnostic workflow.

Purpose of the Study:

  • To introduce Fourier ptychographic microscopy (FPM) as a method to streamline colorectal cancer pathological diagnosis.
  • To demonstrate FPM's capability for large space-bandwidth product imaging and quantitative phase imaging.
  • To show FPM's potential for digital refocusing and removal of artificial markers on pathology slides.

Main Methods:

  • Fourier ptychographic microscopy (FPM) was employed to perform imaging without mechanical operations.
  • The method was validated using typical pathological sections of colorectal cancer.
  • FPM's digital refocusing capability was utilized to digitally remove artificial markers from stained slides.

Main Results:

  • FPM enabled large space-bandwidth product imaging and quantitative phase imaging.
  • The digital refocusing feature of FPM successfully removed artificial markers without manual intervention.
  • Pathologists achieved 100% diagnostic accuracy when using FPM imaging results.

Conclusions:

  • The proposed FPM method significantly simplifies the pathological diagnosis process for colorectal cancer.
  • FPM offers a non-mechanical, efficient approach to imaging and analysis of tumor budding.
  • The cost-effective hardware system for FPM suggests strong potential for widespread adoption in pathology.