Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.6K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.6K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.6K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.6K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

305
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
305
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

992
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
992

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative accuracy of <sup>177</sup>Lu SPECT/CT imaging using ring-shaped CZT versus dual-head NaI systems.

EJNMMI physics·2026
Same author

Radiopharmaceutical Therapy Meets Radiobiology: Implications for Trial Design and DNA Repair Inhibitor Combinations.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
Same author

Factors Influencing Double-Strand Break Focus Biodosimetry for Detection of Internal Low-LET Irradiation in Human Blood Samples.

Radiation research·2026
Same author

Applications of artificial intelligence in nuclear medicine.

Zeitschrift fur medizinische Physik·2026
Same author

Multiple vertebrae improves precision in image-based bone marrow absorbed dose estimation in [<sup>177</sup>Lu]Lu-DOTATATE treatment.

EJNMMI physics·2026
Same author

Current practice in reporting internal dosimetry for [<sup>177</sup>Lu]Lu-DOTATATE therapy: a systematic review.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2026
Same journal

It's PET but not as we know it: radiation protection considerations when using a novel specimen PET-CT scanner during tumour resections for urology and head & neck patients.

EJNMMI physics·2026
Same journal

Data-efficient unsupervised deep learning deformable SPECT/CT registration framework for voxel-level radionuclide therapy dosimetry: validation using clinical <sup>131</sup>I DTC therapy data.

EJNMMI physics·2026
Same journal

Impact of reduced <sup>18</sup>F-MK6240 PET/MR acquisition duration on image quality and tau pathology assessment in patients with cognitive impairment.

EJNMMI physics·2026
Same journal

Optimising [Formula: see text] PET imaging for dosimetry in SIRT: insights from phantom and simulation studies on the Discovery MI scanner.

EJNMMI physics·2026
Same journal

Denoising of 4D dynamic PET images using spatiotemporal regularization with integrated temporal restoration (SPRINTER).

EJNMMI physics·2026
Same journal

Limitations in diagnostics and quantification of small lesions with low uptake in the clinical context of prostate <sup>18</sup>F/<sup>68</sup>Ga-PSMA PET/MRI.

EJNMMI physics·2026
See all related articles

Related Experiment Video

Updated: Sep 18, 2025

Quantifying X-Ray Fluorescence Data Using MAPS
14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

10.9K

Establishing measurement traceability for quantitative SPECT imaging.

Andrew P Robinson1, Kelley M Ferreira2,3,4, Warda Heetun2,5

  • 1National Physical Laboratory, Hampton Road, London, TW11 0LW, UK. andrew.robinson@npl.co.uk.

EJNMMI Physics
|June 23, 2025
PubMed
Summary
This summary is machine-generated.

Establishing measurement traceability for quantitative Single Photon Emission Computed Tomography (SPECT) is crucial for accurate molecular radiotherapy dosimetry. This study demonstrates a method for 177Lu SPECT, achieving 1.6% uncertainty and validating it with organ phantoms.

Keywords:
Measurement uncertaintyQuantitative imagingSPECTTraceability

More Related Videos

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry
07:10

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry

Published on: April 29, 2020

1.8K
O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
06:50

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Published on: November 8, 2019

6.7K

Related Experiment Videos

Last Updated: Sep 18, 2025

Quantifying X-Ray Fluorescence Data Using MAPS
14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

10.9K
3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry
07:10

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry

Published on: April 29, 2020

1.8K
O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
06:50

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Published on: November 8, 2019

6.7K

Area of Science:

  • Medical Imaging
  • Nuclear Medicine
  • Radiotherapy Physics

Background:

  • Quantitative Single Photon Emission Computed Tomography (SPECT) is vital for Molecular Radiotherapy dosimetry.
  • Establishing measurement traceability is essential for data harmonization and inter-site comparison.
  • Uncertainty must be reported at all calibration stages for reliable quantitative measurements.

Purpose of the Study:

  • To demonstrate a manufacturer-independent methodology for establishing measurement traceability in quantitative SPECT.
  • To validate this methodology for the therapeutic radionuclide 177Lu.
  • To present the limitations and capabilities of establishing traceability with standard clinical equipment.

Main Methods:

  • A phantom-based calibration method was used for quantitative 177Lu SPECT.
  • Traceability was established using a calibrated radionuclide calibrator and validated with anthropomorphic organ phantoms.
  • Uncertainty analysis was performed throughout the calibration chain.

Main Results:

  • The dominant uncertainty component originated from the radionuclide calibrator calibration factor (1.57%).
  • The final SPECT Image Calibration Factor uncertainty was 1.6%.
  • Activity recovery in organ phantoms with partial volume correction (PVC) was 96(7)% for kidney and spleen.

Conclusions:

  • A reproducible method for establishing measurement traceability in quantitative 177Lu SPECT was demonstrated.
  • Traceability was successfully established using standard clinical equipment.
  • The study highlights the importance of uncertainty reporting and the impact of corrections like PVC.