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Related Concept Videos

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Updated: May 6, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
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Zwitterionic Poly(sulfobetaine methacrylate) Brushes Functionalized Threads for DNA Extraction from Complex Cell

Xianlong Shi1, Liang Wu1, Ke Ning1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing 400715, China.

Sensors (Basel, Switzerland)
|June 27, 2025
PubMed
Summary
This summary is machine-generated.

Zwitterionic polymer brushes on cotton threads offer a cost-effective and stable method for DNA extraction. This biodegradable material shows comparable performance to commercial kits, enabling PCR amplification from tumor cells.

Keywords:
DNA extractionPCRPSBMA-functionalized threadSI-ATRPpolymer brushsolid-phase extraction

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Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Thread-based analytical devices offer low-cost, portable, and biodegradable solutions for biomolecule detection.
  • Zwitterionic polymers exhibit excellent antifouling properties, crucial for selective biomolecule capture.
  • Current DNA extraction methods often rely on expensive and less environmentally friendly solid-phase extraction techniques.

Purpose of the Study:

  • To develop and evaluate zwitterionic poly(sulfobetaine methacrylate) brushes modified cotton thread (PSBMA@threads) as a novel material for DNA solid-phase extraction.
  • To assess the efficiency, selectivity, and stability of PSBMA@threads for nucleic acid isolation.
  • To compare the performance of PSBMA@threads with commercial DNA extraction kits.

Main Methods:

  • Surface-initiated atom transfer radical polymerization (SI-ATRP) was used to synthesize PSBMA polymer brushes on cotton threads.
  • Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), and Energy-Dispersive X-ray Spectroscopy (EDS) were employed for material characterization.
  • DNA extraction efficiency was evaluated using cell lysates, followed by PCR amplification, and antifouling properties were assessed through protein adsorption and oil repellency tests.

Main Results:

  • Successful functionalization of cotton threads with PSBMA polymer brushes was confirmed by material characterization techniques.
  • PSBMA@threads demonstrated selective DNA capture from complex cell lysates, showing resistance to protein adsorption and oil fouling.
  • Optimized extraction yielded sufficient DNA for successful PCR amplification from tumor cells, with comparable performance to commercial silica columns.
  • The material maintained its DNA extraction capability after six months of ambient storage, indicating high stability.

Conclusions:

  • PSBMA@threads represent a promising, cost-effective, and stable alternative for DNA extraction in analytical applications.
  • The biodegradable nature and excellent antifouling properties of PSBMA@threads enhance their suitability for nucleic acid isolation.
  • This innovative thread-based approach offers significant potential for simplifying and improving DNA purification processes.