Related Experiment Video
Updated: Sep 17, 2025
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
Published on: November 16, 2021
Characterisation of Macrophage Sub-populations in Oral Lichen Planus using CD64 and CD163 - An Evaluative Study
L Surya Surendran1, Anna P Joseph1, Varun B Raghavan Pillai1,2
1Department of Oral & Maxillofacial Pathology and Oral Microbiology, PMS College of Dental Sciences and Research, Kerala University of Health Sciences, Trivandrum, Kerala, India.
Background:
Oral lichen planus (OLP) stands out as one of the prevalent chronic inflammatory conditions affecting the oral cavity, with its aetiology linked to immune-mediated processes. Macrophages play a pivotal role in orchestrating immunological responses within the oral environment. While existing literature suggests significant involvement of macrophages in the progression and persistence of OLP, insights into their specific phenotype expression remain limited.
Aims:
This study aimed to evaluate and compare the expression of M1 and M2 macrophages in reticular and erosive OLP using CD64 and CD163.
Materials And Methods:
This cross-sectional study comprised 15 clinically diagnosed and histopathologically confirmed cases of OLP, along with 15 cases of normal oral mucosa. Densities of CD64 + M1 and CD163 + M2 macrophages were assessed in the epithelial and sub-epithelial areas.
Results:
A significant correlation between macrophage sub-populations in OLP was observed ( P < 0.001). Within OLP, higher densities of CD64 + M1 and CD163 + M2 macrophages were detected in sub-epithelial areas. Notably, M1 macrophage predominated over M2 macrophage sub-populations in OLP, potentially implicating them in the immunopathogenesis.
Conclusions:
These findings hold importance for future investigations targeting therapeutic interventions for OLP.
Insights
This study found that M1 macrophages, involved in inflammation, are more prevalent than M2 macrophages in oral lichen planus (OLP). This suggests M1 macrophages may drive OLP immunopathogenesis, offering therapeutic targets.
Area of Science:
- Immunology
- Oral Pathology
Background:
- Oral lichen planus (OLP) is a common chronic inflammatory oral condition with immune-mediated origins.
- Macrophages are key immune cells, but their specific roles and phenotypes in OLP pathogenesis are not fully understood.
Purpose of the Study:
- To compare M1 and M2 macrophage expression in reticular and erosive OLP.
- To investigate the roles of CD64 (M1 marker) and CD163 (M2 marker) in OLP.
Main Methods:
- Cross-sectional study of 15 OLP cases and 15 controls.
- Quantified CD64+ (M1) and CD163+ (M2) macrophage densities in epithelial and sub-epithelial tissues.
Main Results:
- Significant correlation found between macrophage subpopulations in OLP (P < 0.001).
- Higher densities of both M1 and M2 macrophages were observed in the sub-epithelial areas of OLP lesions.
- M1 macrophages predominated over M2 macrophages in OLP, suggesting a pro-inflammatory role.
Conclusions:
- M1 macrophage predominance in OLP implicates them in the disease's immunopathogenesis.
- Findings provide a basis for future research into targeted OLP therapies.
More Related Videos
11:25Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
Published on: December 26, 2013
08:37Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces
Published on: December 6, 2024