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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
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Fast Tissue Clearing and Volume Imaging Techniques for Anatomy.

Clément Ricard1, Doriane Hazart1, Brigitte Delhomme1

  • 1Université Paris Cité, SPPIN, Saint-Pères Paris Institute for the Neurosciences, CNRS, Paris, France.

Microscopy Research and Technique
|June 28, 2025
PubMed
Summary
This summary is machine-generated.

Microscopy now images whole organs in 3D using advanced tissue clearing and 3D microscopy techniques, overcoming limitations of traditional 2D methods for detailed anatomical studies.

Keywords:
anatomopathologyanatomylight‐sheet microscopythree‐dimensional microscopytissue clearingtwo‐photon microscopy

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Area of Science:

  • Biological Imaging
  • Cellular Biology
  • Anatomical Pathology

Background:

  • Traditional optical microscopy is limited to thin samples, hindering 3D analysis.
  • Stereology offers 3D interpretation from 2D slices but is laborious and error-prone.
  • Advancements are needed to image intact biological tissues in three dimensions.

Purpose of the Study:

  • To review modern tissue-clearing methods.
  • To describe advanced 3D microscopy techniques.
  • To highlight combined approaches for subcellular imaging of intact tissues.

Main Methods:

  • Tissue clearing techniques to render samples transparent.
  • Advanced microscopy, including confocal, two-photon, and light-sheet microscopy.
  • Integration of clearing and 3D imaging for intact organ analysis.

Main Results:

  • Tissue clearing enables deep imaging of previously opaque samples.
  • 3D microscopy techniques achieve significant imaging depths.
  • Combined methods allow subcellular resolution in cleared whole organs.

Conclusions:

  • Modern microscopy has overcome the 3D imaging frontier for biological samples.
  • Tissue clearing and 3D microscopy offer new perspectives in anatomy and pathology.
  • These integrated techniques facilitate detailed subcellular analysis of intact organs.