Protein synthesis in cells infected by chilo iridescent virus (iridovirus, type 6)

Synthesis of the polypeptides induced in CIV-infected cells was studied using radiolabelled methionine in a permissive cell line of Choristoneura fumiferana. Analysis of labelled cell extracts by high resolution polyacrylamide gel electrophoresis (PAGE) revealed the sequential appearance of at least 28 structural and non-structural polypeptides in permissive conditions. This was confirmed by analysis of protein synthesis in non-permissive cell lines of Lymantria dispar, Aedes albopictus or by the use of protein, RNA and DNA synthesis inhibitors in the permissive cell line.