Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

92.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.7K
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

14.4K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
14.4K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

15.9K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterisation of the Novel HLA-DRB3*01:01:27 Allele Using Third-Generation Sequencing Methods.

HLA·2026
Same author

Characterisation of the Novel HLA-DQA1*01:02:32 Allele Using Third-Generation Sequencing Methods.

HLA·2026
Same author

Characterisation of the Novel HLA-C*12:459 Allele Using Third-Generation Sequencing Methods.

HLA·2026
Same author

Third-Generation Nanopore Sequencing for Post-Transplant Chimerism Monitoring.

HLA·2026
Same author

Minimal immune response after transplantation of a cryopreserved human amniotic membrane on the ocular surface.

Materials today. Bio·2026
Same author

Reassessing the cryopreserved human amniotic membrane's low immunogenicity due to advances in histocompatibility.

Materials today. Bio·2025

Related Experiment Video

Updated: Sep 16, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

29.6K

Characterisation of the Novel HLA-A*29:198 Allele Using New Next-Generation Sequencing Methods.

Théo Marchal1, Lucas Hubert1, Coralie Frassati1

  • 1Immunogenetics Laboratory, Etablissement Français du Sang PACA Corse, Marseille, France.

HLA
|July 9, 2025
PubMed
Summary

This study identifies a novel Human Leukocyte Antigen (HLA) allele, HLA-A*29:198. It differs from a known allele by a single nucleotide substitution, impacting the HLA immune response.

Keywords:
HLA‐A*29:198HLANGSnew methodsnovel allele

More Related Videos

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.9K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.1K

Related Experiment Videos

Last Updated: Sep 16, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

29.6K
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.9K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.1K

Area of Science:

  • Immunogenetics
  • Molecular biology
  • Human leukocyte antigen system

Background:

  • The Human Leukocyte Antigen (HLA) system plays a critical role in immune response and transplantation.
  • Allelic variations within HLA genes contribute to diverse immune capabilities and disease susceptibility.
  • Accurate characterization of novel HLA alleles is essential for immunological research and clinical applications.

Purpose of the Study:

  • To characterize a newly identified Human Leukocyte Antigen (HLA) allele, designated HLA-A*29:198.
  • To elucidate the specific genetic difference between HLA-A*29:198 and a previously described allele, HLA-A*29:02:01:01.

Main Methods:

  • Nucleotide sequencing of the HLA-A gene.
  • Comparative analysis of DNA sequences to identify variations.
  • Bioinformatic tools for allele nomenclature and classification.

Main Results:

  • The novel allele HLA-A*29:198 was identified and formally designated.
  • A single nucleotide substitution was detected in codon 127 within exon 3 when comparing HLA-A*29:198 to HLA-A*29:02:01:01.
  • This specific substitution represents the sole genetic divergence between the two alleles.

Conclusions:

  • HLA-A*29:198 represents a distinct genetic variant within the HLA-A locus.
  • The identified nucleotide substitution provides a precise molecular definition for this new allele.
  • Further studies are warranted to investigate the potential functional and clinical implications of HLA-A*29:198 in immune responses.