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Related Experiment Video

Updated: Sep 16, 2025

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

3.3K

TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation.

Fanju W Meng1, Paige Schneider2, Xiaolu Wei1

  • 1Department of Biological Sciences, Advanced Environmental Research Institute, University of North Texas 1155 Union Circle, Denton, TX 76203, USA.

Cell Reports Methods
|July 9, 2025
PubMed
Summary

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TransTag is a new method for identifying where genes insert into zebrafish DNA. This tool helps researchers understand gene expression and improve transgenic studies.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Zebrafish are a key model organism in developmental biology due to facile transgenesis.
  • Lack of transgene insertion site mapping complicates interpretation of gene expression and allelic variation.
  • Current methods for insertion site identification are complex and require bioinformatics expertise.

Purpose of the Study:

  • To introduce TransTag, a simple and efficient method for identifying Tol2-based transgene insertion sites in zebrafish.
  • To provide researchers with a low-cost, accessible tool for transgene mapping.
  • To enhance the precision and utility of zebrafish transgenesis studies.

Main Methods:

  • Utilizes Tn5 transposase-mediated tagmentation for streamlined insertion site identification.
Keywords:
CP: geneticsR ShinyTn5Tol2tagmentationtransgenesistranspositionzebrafish

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Last Updated: Sep 16, 2025

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  • Does not require alignment of raw sequencing data.
  • Includes a detailed protocol, data analysis guide, and a user-friendly Shiny app.

    • Main Results:

    • TransTag enables straightforward and efficient identification of transgene insertion sites.
    • The method bypasses the need for complex bioinformatics analysis.
    • Provides chromosome-specific information on transgene locations.

    Conclusions:

    • TransTag is a valuable tool for zebrafish transgenesis research.
    • Enhances precision by providing essential genetic location data.
    • Lowers the barrier for researchers to map transgene insertion sites.