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G4-SLSELEX-Seq-driven discovery of a G4-specific targeting L-RNA aptamer with unique structural features
Tian-Ying Wu1, Chun Kit Kwok1,2
1Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong, Hong Kong SAR 999077, China. ckkwok42@cityu.edu.hk.
Organic & Biomolecular Chemistry
|July 10, 2025
Abstract:
We integrated sequence-guided library design into G4-SLSELEX-Seq, expanding its application to aptamer selection against DNA G-quadruplexes (dG4s). This strategy identified L-Apt.G3, the first L-RNA aptamer exhibiting low nanomolar binding affinity and dual-specificity binding to parallel and antiparallel G4s through a unique binding motif. Additionally, L-Apt.G3 can competitively disrupt dG4-protein interactions.

