CPF Induces GC2_spd Cell Injury via ROS/AKT/Efcab6 Pathway

Area of Science:

• Environmental Toxicology
• Reproductive Biology
• Molecular Mechanisms

Background:

• Chlorpyrifos (CPF) is a widely used insecticide with known reproductive toxicity.
• Pesticide exposure presents growing concerns for male reproductive health.

Purpose of the Study:

• To investigate the molecular mechanisms of male reproductive decline induced by Chlorpyrifos (CPF).
• To identify potential therapeutic targets for CPF-induced male infertility.

Main Methods:

• Utilized GC2_spd cells for in vitro studies.
• Employed flow cytometry, qRT-PCR, Western blot, RNA sequencing, and bioinformatics analysis.
• Investigated the role of reactive oxygen species (ROS) and the ROS/AKT/Efcab6 pathway.

Main Results:

• CPF significantly affected cell viability, cell cycle, apoptosis, and ROS accumulation in GC2_spd cells.
• RNA sequencing identified 626 differentially expressed genes, including Efcab6, Nox3, and Cmpk2.
• CPF induces male reproductive damage through the ROS/AKT/Efcab6 pathway, involving PI3K-AKT signaling and glutamine metabolism.

Conclusions:

• CPF exposure leads to male reproductive damage via the ROS/AKT/Efcab6 pathway.
• Elucidates molecular targets and mechanisms for CPF-induced male infertility.
• Provides a theoretical basis for preventing pesticide-induced reproductive harm.