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Comparison of Polygonatum sibiricum Polysaccharides from Different Extraction Methods
Yan Chen1, Hanchen Du1, Wenjie Qu1
1Key Laboratory of Public Nutrition and Health, National Health Commission of the People's Republic of China, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
The ultrasound-assisted extraction-deep eutectic solvent method yields the highest Polygonatum sibiricum Polysaccharide. Further purification is not recommended as it does not improve, and may decrease, antioxidant activity.
Area of Science:
- Biochemistry
- Natural Product Chemistry
- Food Science
Background:
- Polygonatum sibiricum Polysaccharide (PSP) is a valuable compound with potential health benefits.
- Previous research indicated ultrasound-assisted extraction-deep eutectic solvent (UAE-DES) method offers high extraction yield for PSP.
- Uncertainty exists regarding the performance and impact of purification on PSP extracted via UAE-DES compared to other methods.
Purpose of the Study:
- To compare the performance of PSP extracted using different methods, including UAE-DES.
- To evaluate the effect of separation and purification on PSP performance.
- To determine the optimal extraction and processing strategy for PSP.
Main Methods:
- Extraction of PSP using UAE-DES and other unspecified methods.
- Comparison of extraction yield, structural composition, and antioxidant activity (DPPH and ABTS assays).
- Analysis of PSP before and after separation and purification.
Main Results:
- UAE-DES method achieved the highest extraction yield (45.08%).
- Structural compositions were similar across methods; purified PSP is a neutral polysaccharide.
- PSP from UAE-DES showed significantly higher antioxidant activity, which was further enhanced after purification.
Conclusions:
- UAE-DES method maximizes PSP extraction yield, structural integrity, and antioxidant potential.
- Separation and purification of PSP extracted via UAE-DES are not necessary and may not be beneficial.
- This study provides an optimized technique for PSP extraction, supporting its application in the health product industry.
