Advances in Fungal Promoter Engineering for Enhancing Secondary Metabolite Biosynthesis
1School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China.
Biotechnology Journal
|July 14, 2025
Summary
Promoter engineering enhances fungal secondary metabolite (SM) production by fine-tuning gene transcription. This review covers techniques like promoter replacement and optimization for increased SM yields.
Area of Science:
- Molecular Biology
- Biotechnology
- Mycology
Background:
- Promoter engineering is crucial for regulating gene transcription in fungi.
- It is widely used to enhance the expression of secondary metabolite (SM) biosynthetic gene clusters (BGCs).
- This strategy has significantly advanced SM production over the last two decades.
Purpose of the Study:
- To provide a comprehensive overview of recent advances in fungal promoter engineering techniques.
- To discuss the application of these techniques in enhancing SM production.
- To explore current challenges and future prospects in the field.
Main Methods:
- Review of recent literature on promoter engineering in fungi.
- Analysis of promoter replacement, optimization, and combinatorial methods.
- Discussion of applications in SM production and metabolic regulation.
Main Results:
- Promoter engineering offers powerful strategies for controlling gene expression in fungi.
- Various techniques have been successfully applied to increase SM yields.
- Significant progress has been made in optimizing BGC expression.
Conclusions:
- Promoter engineering is a key tool for enhancing fungal secondary metabolite production.
- Continued research into novel techniques and optimization strategies is essential.
- Addressing current challenges will pave the way for precise metabolic regulation in fungi.
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