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Updated: Jul 21, 2026

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Published on: October 21, 2010
Optimization of in vitro Biofilm Growth of Candida albicans
Mazen Abdulghani1, Gajanan Zore2
1School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, MS 431606 India.
Abstract:
Candida albicans cells growing under biofilm form exhibit exceptional resistance towards anti-fungal agents and host immune responses. In this report, we have investigated variables that affect C. albicans biofilm development. Our observations suggest that factors like inoculum density, adhesion time and media composition play important roles in regulating biofilm formations and inoculum density of 1 × 107 cells/mL, adhesion for 90 min, and RPMI 1640 were the optimal conditions.

