Multifunctional PLGA/collagen/zeolitic imidazolate framework-8 composite nanofibrous membranes for guided bone...

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Nanotechnology

Background:

• Guided bone regeneration (GBR) is crucial for treating maxillofacial defects.
• Current GBR membranes face challenges in enhancing osteoinduction and antimicrobial resistance.
• Developing advanced composite membranes is essential for improved bone defect therapy.

Purpose of the Study:

• To develop and characterize novel poly(lactide-co-glycolide) (PLGA) nanofibrous membranes reinforced with collagen (Col) and zeolitic imidazolate framework-8 (ZIF-8).
• To evaluate the biocompatibility, osteogenic potential, and antibacterial efficacy of the composite membranes.
• To assess the in vivo bone regeneration capacity of the developed membranes in a rat cranial defect model.

Main Methods:

• Fabrication and material characterization of PLGA/Col/ZIF-8 composite nanofibrous membranes.
• In vitro assessment of biocompatibility, osteogenic differentiation, and antibacterial activity.
• In vivo evaluation of bone regeneration using Micro-CT and histological analysis in a rat calvarial defect model.

Main Results:

• Optimized PLGA/Col/ZIF-8 membranes (100:3 ratio, 1 wt% ZIF-8) exhibited superior tensile strength and biocompatibility.
• Sustained zinc ion (Zn²⁺) release from ZIF-8 demonstrated dual effects: stimulating osteogenic differentiation and inhibiting bacterial infection.
• In vivo studies confirmed significant bone regeneration in rat calvarial defects treated with the composite membrane.

Conclusions:

• The developed PLGA/Col/ZIF-8 composite nanofibrous membranes show significant potential for guided bone regeneration.
• These membranes offer a promising strategy for enhancing osteoinduction and providing antimicrobial resistance in bone defect therapies.
• The findings support the clinical applicability of these advanced biomaterials for maxillofacial reconstruction.