A lipid plug affects K_2P6.1(TWIK-2) function

Lipids are integral to ion channel function yet delineating mechanisms by which they affect function remains challenging. Within the K_2P family of leak potassium channels^1-3, observation of tubular densities interpreted as alkyl chains occupying lateral fenestrations linking the pore and bilayer^4-8 raised the possibility that lipid access from the bilayer acts as a regulatory mechanism^4-7. Here, we present cryo-electron microscopy (cryo-EM) structures of the human leak potassium channel K_2P6.1 (TWIK2)^9-11 and mutants in nanodisc and detergent environments that reveal an unusual conformation in the first selectivity filter (SF1) and a pair of two-chain lipids within the channel cavity (denoted the 'lipid plug'). The chains of each plug lipid occupy separate binding sites that laterally extend to the bilayer from the channel cavity. One, the upper leg, matches the previously identified alkyl chain binding site^4-8,12. The second, the lower leg, occupies a fenestration common with K_2P1.1 (TWIK1)¹³. Together, they demonstrate a bidentate means to coordinate each plug lipid that offers a reinterpretation of previous observations. Structures of a K_2P6.1 (TWIK2) mutant that directs the channel to the plasma membrane¹⁴ and an R257A mutant that increases function yield plugged and unplugged forms. Notably, the R257A plugged form shows a change in lipid plug position, indicating a key role for this residue in lipid binding. Together, our data suggest that occupation of the central cavity by the lipid plug serves as a mechanism to render the TWIK channels inactive and points to the importance of lipid plug removal to create an ion permeable pore. Such a mechanism could provide a potent way for limiting the leak function of K_2Ps based on cellular location or other contextual factors.