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Related Concept Videos

Open Angle Glaucoma: Treatment01:27

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...

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Related Experiment Video

Updated: Jun 26, 2026

Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects
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Inhibitory Effects of 3',4'-Dihydroxyflavonol in a Rabbit Model of Minimally Invasive Bleb Surgery With PreserFlo

Zoe Pasvanis1,2, Roy C K Kong1,2,3, Elsa C Chan1,2,3

  • 1Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.

Clinical & Experimental Ophthalmology
|July 16, 2025
PubMed
Summary

3',4'-Dihydroxyflavonol (DiOHF) effectively reduced scarring after PreserFlo MicroShunt (PFMS) surgery in rabbits. This novel compound shows potential as a safer antifibrotic agent compared to Mitomycin C (MMC).

Keywords:
fibroblastsglaucomainflammationminimally invasive glaucoma surgeryoxidative stresswound healing

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Area of Science:

  • Ophthalmology
  • Glaucoma Surgery
  • Wound Healing

Background:

  • Minimally invasive bleb surgery (MIBS) success, like PreserFlo MicroShunt (PFMS), is hindered by post-operative bleb fibrosis.
  • Current antifibrotic therapy uses off-label cytotoxic agents such as Mitomycin C (MMC).
  • This study evaluates 3",4"-Dihydroxyflavonol (DiOHF) as a novel antifibrotic agent in a rabbit model of PFMS surgery.

Purpose of the Study:

  • To investigate the anti-scarring properties of DiOHF.
  • To compare DiOHF's efficacy against vehicle control and Mitomycin C (MMC).
  • To assess DiOHF's impact on fibrosis, oxidative stress, inflammation, and fibroblast activity post-PFMS.

Main Methods:

  • Unilateral MIBS with PFMS was performed on 15 New Zealand white rabbits.
  • Rabbits were randomized into three groups: vehicle, DiOHF, or MMC.
  • Bleb morphology was assessed clinically and histologically (collagen, αSMA, oxidative stress, inflammation) on Day 14.

Main Results:

  • DiOHF treatment resulted in decreased vimentin, αSMA, and 3-nitrotyrosine expression compared to vehicle.
  • DiOHF blebs showed reduced CD45 expression (inflammation) and less collagen accumulation than vehicle and MMC groups.
  • MMC-treated blebs exhibited increased size and ischemia compared to DiOHF and vehicle groups.

Conclusions:

  • DiOHF effectively inhibited scarring after PFMS implantation in rabbit eyes.
  • DiOHF reduced local oxidative stress, inflammation, and fibroblastic activity.
  • DiOHF presents a potentially safer and more effective alternative to MMC for wound modulation in glaucoma surgery.