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Paper-Droplet Hybrid Strategy for Highly Selective Blood Plasma Separation in Multiplex Diagnostics.

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This study introduces a new hybrid microfluidic device for fast and efficient blood plasma separation (≥98%) from small samples (6 μL). The technology enables automated biomarker detection for improved diagnostics at the point-of-care.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Conventional microfluidic paper-based analytical devices (μPADs) face limitations in blood microsampling efficiency and downstream detection.
  • Need for rapid, high-purity plasma separation and automated biomarker analysis from minimal blood volumes.

Purpose of the Study:

  • To develop a novel hybrid sessile-droplet and paper microfluidic technique for efficient blood plasma separation and automated biomarker detection.
  • To overcome limitations of existing μPADs by integrating passive microfluidic strategies for enhanced analytical precision.

Main Methods:

  • Utilized a hybrid approach combining sessile-droplet and paper microfluidics.
  • Employed evaporation-driven natural convection for enhanced fluid mixing.
  • Leveraged capillary-driven plasma extraction for selective, high-purity separation without external actuation.
  • Integrated automated downstream biomarker detection with a custom application (GPS App.) for real-time quantification.

Main Results:

  • Achieved rapid plasma separation in approximately 70 seconds with high efficiency (≥98%) from a small blood sample volume (∼6 μL).
  • Demonstrated high sensitivity, reproducibility, and multiplexing capabilities.
  • Attained ≥90% analytical accuracy for simultaneous detection of glycemic, albuminic, and diabetic conditions.
  • Ensured unbiased diagnostics through automated, real-time quantification via the GPS App.

Conclusions:

  • The developed hybrid microfluidic technique offers a scalable, cost-effective, and highly efficient biosensing platform.
  • This integrated approach enhances analytical precision and overcomes limitations of individual microfluidic systems.
  • The technology is suitable for unbiased diagnostics in extreme point-of-care settings.