Related Experiment Video
Updated: Sep 15, 2025
16:15
Mouse Kidney Transplantation: Models of Allograft Rejection
Published on: October 11, 2014
20.9K
Biopsy-Based Transcriptomics Support Rejection Monitoring Through Repeated Kidney Allograft Biopsies.
Lukas Weidmann1, Dusan Harmacek1, Ariana Gaspert2
1Division of Nephrology, University Hospital of Zurich, Switzerland.
Kidney International Reports
|July 18, 2025
Summary
Biopsy transcriptomics detects early kidney transplant rejection signals missed by histology, even after treatment. This molecular approach monitors rejection progression and treatment effectiveness in kidney allografts.
Area of Science:
- Nephrology
- Transplant immunology
- Genomics
Background:
- Kidney allograft biopsies are crucial for diagnosing rejection.
- Histopathology may miss subthreshold rejection signals or treatment responses.
Purpose of the Study:
- To evaluate biopsy-based transcriptomics for detecting rejection in kidney allografts.
- To assess transcriptomics' ability to monitor treatment response and rejection phenotyping.
Main Methods:
- Analysis of 80 kidney allograft biopsy series (baseline and follow-up).
- Assessment using histopathology (Banff classification) and Molecular Microscope Diagnostic System (MMDx).
Main Results:
- Baseline biopsies showed significant molecular rejection (55% confirmation).
- Follow-up biopsies detected histological rejection in 36% of previously "rejection-free" cases.
- Transcriptomics identified higher rejection and AMR probabilities in cases progressing to rejection.
- Molecular and histological scores improved in treated mixed AMR/TCMR cases, with varied outcomes.
Conclusions:
- Biopsy transcriptomics detects molecular signals of rejection missed by histology.
- Transcriptomics serves as a monitoring tool for antirejection treatment effectiveness.
Related Concept Videos
01:28Kidney Transplant I: Introduction
60
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
60
01:26Kidney Transplant II: Surgical Procedure
77
Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living...
77
01:16Kidney Transplant III: Nursing Management
83
Postoperative Nursing Management for Kidney Transplant PatientsPostoperative nursing management care includes monitoring the surgical site, encouraging early movement, and promoting lung health through breathing exercises. Nurses also administer prescribed medications like H2-blockers, such as famotidine, or proton pump inhibitors, like omeprazole, to help prevent gastrointestinal ulcers and bleeding. Fungal infections in the mouth and bladder can result from immunosuppressive and antibiotic...
83