Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Immune checkpoint inhibitor therapy after tumor-infiltrating lymphocytes in unresectable melanoma.

Journal for immunotherapy of cancer·2026

Same author

Deconvolving SARS-CoV-2 mRNA vaccine impact on immunotherapy-related survival.

Cancer discovery·2026

Same author

Diagnostic delay in histiocytic neoplasms and its association with local resource deprivation.

Haematologica·2026

Same author

Enhanced Telehealth in Prostate Cancer.

JAMA network open·2026

Same author

T-Cell Clonal Expansion in Peripheral Blood Following Interventional Radiology Procedures for Metastatic Liver Cancer.

Cancers·2026

Same author

Perspective Editorial: Assessment of Survival Benefit from Sentinel Node Biopsy for Melanoma: A Systematic Review and Meta-analysis.

Annals of surgical oncology·2026

Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026

Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026

Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026

Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026

Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026

Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 14, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Scalable topic modelling decodes spatial tissue architecture for large-scale multiplexed imaging analysis.

Xiyu Peng^1,2, James W Smithy³, Mohammad Yosofvand⁴

¹Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, USA. pengx@stat.tamu.edu.

Nature Communications

|July 18, 2025

Summary

This summary is machine-generated.

SpatialTopic is a new computational method that analyzes large multiplexed tissue images to reveal spatial patterns. This approach enhances understanding of tumor microenvironments and disease progression.

More Related Videos

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

Published on: May 12, 2019

Related Experiment Videos

Last Updated: Sep 14, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

Published on: May 12, 2019

Area of Science:

Computational Biology
Bioinformatics
Pathology

Background:

Multiplexed tissue imaging advances tumor microenvironment research.
Analyzing large whole-slide images is computationally intensive.
Limited methods exist for integrative cross-image analysis.

Purpose of the Study:

Introduce SpatialTopic, a spatial topic model for decoding tissue architecture from multiplexed images.
Integrate cell type and spatial data to identify recurrent, biologically meaningful spatial patterns ('topics').

Main Methods:

Developed SpatialTopic, a spatial topic model.
Integrated cell type and spatial information.
Benchmarked across diverse spatial transcriptomic and proteomic imaging platforms and tissue types.

Main Results:

SpatialTopic is scalable to large images with high precision and interpretability.
Identified biologically and clinically significant spatial topics, including tertiary lymphoid structures.
Tracked spatial changes during disease progression.

Conclusions:

SpatialTopic offers an efficient and broadly applicable method for analyzing large-scale multiplexed imaging datasets.
Enhances understanding of spatial tissue architecture in relation to disease.