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A quorum sensing-controlled type I CRISPRi toolkit for dynamically regulating metabolic flux

Han Zheng1, Chengyao Mao1, Siyao Chen1

  • 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.

Nucleic Acids Research
|July 19, 2025
PubMed
Summary

We developed a novel quorum sensing-controlled CRISPR interference (QICi) toolkit for dynamic metabolic engineering. This tool enhances microbial production of valuable compounds like d-pantothenic acid and riboflavin.

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