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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Epigenetic Regulation01:37

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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Related Experiment Video

Updated: Sep 14, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues
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Exploring Epigenetic Ageing Using Direct Methylome Sequencing.

Elena-Cristina Găitănaru1, Roua Gabriela Popescu1,2, Andreea-Angelica Stroe3

  • 1Independent Research Association, 012416 Bucharest, Romania.

Epigenomes
|July 23, 2025
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Summary

Nanopore sequencing offers a new method for epigenetic ageing research by profiling DNA methylation. This approach provides richer data than traditional methods, advancing studies on ageing and interventions.

Keywords:
DNA methylationageing related CpGsepigenetic clocknanopore adaptive samplingnanopore epigenetic clock

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Area of Science:

  • Genomics
  • Epigenetics
  • Bioinformatics

Background:

  • Nanopore sequencing advances enable single-base resolution DNA methylation studies.
  • Epigenetic ageing research has not fully utilized nanopore sequencing capabilities.

Purpose of the Study:

  • To present a novel framework for profiling and interpreting age-associated DNA methylation patterns using nanopore sequencing.
  • To establish nanopore sequencing as a tool for next-generation epigenetic ageing research.

Main Methods:

  • Utilizing nanopore sequencing for native DNA methylation profiling.
  • Developing a framework to interpret age-associated methylation patterns.

Main Results:

  • Long-read sequencing captures full CpG context and repetitive genomic regions.
  • Eliminates the need for bisulfite conversion, unlike array-based methods.
  • Compatible with the latest reference genomes, offering enhanced accuracy.

Conclusions:

  • Nanopore sequencing is a powerful tool for epigenetic ageing studies.
  • Provides a scalable and biologically rich platform for monitoring anti-ageing interventions.
  • Supports longitudinal ageing studies with comprehensive data.