Antimicrobial Efficient Biological Adhesive for Infected Wound Repair: Robust Tissue Adhesion, Broad-Spectrum...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Infectious Diseases

Background:

• Chronic infected wounds present challenges due to inflammation and persistent bacterial biofilms, hindering healing and causing scarring.
• Existing bioadhesives often lack adaptability and multifunctionality, potentially worsening tissue damage.

Purpose of the Study:

• To engineer a novel protein-based bioadhesive with enhanced microenvironmental adaptability and multifunctionality for chronic infected wound treatment.
• To evaluate the adhesive properties, antibacterial efficacy, and regenerative potential of the engineered bioadhesive.

Main Methods:

• Fabrication of a protein-based bioadhesive by integrating Lanmodulin (LanM) and arginine-rich elastin-like (Rprotein) modules.
• Incorporation of lanthanide ions (Ln³⁺) to induce conformational changes and enhance material properties.
• Assessment of adhesive strength, antibacterial activity against biofilms, and in vivo wound healing in murine models.

Main Results:

• The engineered bioadhesive demonstrated superior adhesive strength compared to commercial products.
• Achieved rapid and potent antibacterial activity, eliminating 99.9% of bacteria by disrupting biofilms and inducing reactive oxygen species.
• Significantly promoted angiogenesis and collagen remodeling, resulting in 97% wound closure in murine models.

Conclusions:

• The developed biphasic bioadhesive offers a combination of mechanical robustness, rapid antibacterial action, and pro-regenerative capabilities.
• Represents a transformative approach for treating chronic infected wounds with high potential for clinical translation in advanced wound care.