In-Vitro Bioactive Silver Nanoparticles Synthesized from Halodule uninervis Plant Extract for Multifunctional Drug...

Area of Science:

• Nanotechnology
• Materials Science
• Biotechnology

Background:

• Silver nanoparticles (AgNPs) are increasingly explored for biomedical applications.
• Green synthesis methods offer sustainable alternatives for nanoparticle production.
• Seagrass extracts present a potential source for eco-friendly AgNPs synthesis.

Purpose of the Study:

• To synthesize silver nanoparticles using seagrass aqueous extract (HU).
• To characterize the synthesized HU-AgNPs using various spectroscopic and microscopic techniques.
• To evaluate the antibacterial, antioxidant, and antidiabetic activities of HU-AgNPs.

Main Methods:

• Synthesis of AgNPs using seagrass (HU) extract and silver nitrate.
• Characterization via UV-vis spectrophotometry, FTIR, SEM, XRD, and AFM.
• Assessment of antibacterial activity against Gram-negative pathogens.
• Evaluation of antioxidant capacity using ABTS, DPPH, H2O2, NO, and superoxide scavenging assays.
• Determination of antidiabetic potential through α-amylase and α-glycosidase inhibition assays.

Main Results:

• Successful synthesis of HU-AgNPs confirmed by color change and UV-vis spectroscopy.
• FTIR confirmed the presence of functional groups, SEM validated spherical morphology, and XRD revealed crystal structure.
• HU-AgNPs demonstrated significant antibacterial activity against Gram-negative bacteria.
• Strong antioxidant activity was observed, particularly in ABTS radical scavenging.
• Effective inhibition of α-amylase and α-glycosidase indicated antidiabetic potential.

Conclusions:

• Seagrass-mediated synthesis provides a viable route to produce functional AgNPs.
• HU-AgNPs exhibit promising antibacterial, antioxidant, and antidiabetic properties.
• Further in vivo studies are warranted to explore the therapeutic potential of HU-AgNPs for drug development.