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miRNA-29c-3p Activates the JAK2/STAT3 Signaling Pathway by Down-Regulating SOCS3 to Promote Pathological Angiogenesis

Xiao-Mei Chen1, Min Wen1, Rong Wu2

  • 1School of Medicine, Zunyi Medical University, Zunyi, Guizhou, China.

Annals of Clinical and Laboratory Science
|August 1, 2025
PubMed
Abstract

Insights

MicroRNA-29c-3p promotes diabetic retinopathy (DR) by targeting SOCS3 and activating the JAK2/STAT3 pathway. Inhibiting miRNA-29c-3p may offer a therapeutic strategy for DR.

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genetics

Background:

  • Diabetic retinopathy (DR) is a microvascular complication of diabetes.
  • Understanding the molecular mechanisms of DR progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of microRNA-29c-3p (miRNA-29c-3p) in regulating Suppressors of Cytokine Signaling 3 (SOCS3) in diabetic retinopathy (DR).
  • To elucidate the potential mechanisms by which miRNA-29c-3p influences DR progression.

Main Methods:

  • Established a DR cell model using human retinal microvascular endothelial cells (hRMECs) exposed to high glucose.
  • Utilized transfection to down-regulate miRNA-29c-3p and SOCS3 expression.
  • Analyzed cell migration, angiogenesis, pro-inflammatory cytokine release, and JAK2/STAT3 pathway activation.

Main Results:

  • High glucose increased miRNA-29c-3p and decreased SOCS3 expression in hRMECs.
  • Inhibiting miRNA-29c-3p suppressed hRMEC migration, angiogenesis, and pro-inflammatory cytokine release, effects partially reversed by SOCS3 knockdown.
  • miRNA-29c-3p directly targets SOCS3 mRNA, and SOCS3 knockdown activates the JAK2/STAT3 pathway, which can be mitigated by reducing miRNA-29c-3p.

Conclusions:

  • miRNA-29c-3p promotes DR progression by negatively regulating SOCS3.
  • The mechanism involves the activation of the JAK2/STAT3 signaling pathway.
  • Targeting miRNA-29c-3p or SOCS3 may represent a therapeutic approach for DR.
Keywords:
Diabetic retinopathyangiogenesisinflammatory responsemiRNA-29c-3psuppressors of cytokine signaling 3

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