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Updated: Sep 13, 2025
Author Spotlight: Advancements in iPSCs and Genetic Disease Research
Published on: October 20, 2023
Proteomic Comparison of Conditioned Media from Mesenchymal Stem Cells of Different Human Tissues: Implications for
Atsushi Terunuma1, Chun Kai Chiu2, Tatsuya Sekine2
1N2 Clinic Yotsuya, Tokyo, Japan.
Objective:
Mesenchymal stem cells (MSCs) suppress inflammation and promote tissue repair via paracrine factors. MSC-conditioned medium (MSC-CM), rich in these factors, shows promise as a cell-free therapy. This study explored the protein profiles of MSC-CMs from different human tissues (dental pulp, adipose tissue, umbilical cord, and placenta) to assess variations and therapeutic potential.
Methods:
MSCs were cultured from dental pulp, adipose tissue, umbilical cord, and placenta, and MSC-CMs were collected. Proteomic analysis using LC-MS/MS identified and quantified proteins, followed by bioinformatic analysis.
Results:
A total of 924 secreted proteins were identified in MSC-CMs from the four tissue sources. Extracellular matrix (ECM) signatures were prominent across all MSC-CMs. MSC-CM from adipose tissue had the highest levels of skin care-related proteins. Neuronal growth-related proteins were most abundant in umbilical cord and placental MSC-CMs, while wound healing proteins were prominent in dental pulp MSC-CM.
Conclusions:
MSC-CMs from different tissues exhibit distinct protein profiles, while sharing common ECM signatures. These findings suggest that MSC-CM could be used for specific applications such as neurodegenerative diseases and wound healing, depending on the tissue source. Further in vivo research is needed to explore their clinical relevance.
