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A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published on: July 18, 2011
Bioinformatics analysis identifies Mot2 protein as a potential regulator of autophagy in Saccharomyces cerevisiae
Emmanuella Wesome Avogo1, Nicholas A Burlingame1, Swaroopa Badenahalli Narasimhaiah1
1Biological Sciences, Oakland University, Rochester, Michigan, United States.
Abstract:
Macroautophagy/autophagy is a conserved mechanism of cellular degradation and recycling. Autophagy is a multi-step process that must be precisely regulated at multiple levels (transcriptional, post-transcriptional, translational, and post-translational). However, there is a gap in our understanding of the molecular details of these mechanisms. Therefore, characterization of factors modulating autophagy and autophagy-related ( ATG ) genes is an important area for investigation. Here, we used a bioinformatics approach to screen 37 yeast ATG genes using the YEASTRACT database to identify potential regulators. Through our selection criteria, we discovered one novel factor-Mot2p. Our findings support that the yeast Mot2 protein is a regulator of cell survival, ATG8 , and potentially, autophagy.
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