Related Experiment Video

Updated: Sep 12, 2025

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
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Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

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Increased Expression of RCN1P2, TPM3P9, and HSP90AB3P as Non-Coding RNA in Gastric Cancer Linked to Proliferative,

Ensieh Sagheb Sadeghi1,2, Zahra Amrollahy Bioky2,3, Mahsa Hokmabadi2,4

  • 1Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.

Iranian Journal of Public Health
|August 6, 2025
PubMed
Abstract

Insights

Altered pseudogene expression, including RCN1P2, TPM3P9, and HSP90AB3P, is linked to gastric cancer (GC) progression. These pseudogenes influence cell proliferation, inflammation, and metastasis through the competing endogenous RNA network, impacting patient survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Gastric cancer (GC) pathogenesis involves complex molecular alterations.
  • Pseudogenes, non-coding RNAs, are increasingly recognized for their roles in cancer development.

Purpose of the Study:

  • Identify pseudogenes with altered expression in GC.
  • Investigate their role in GC development via the competing endogenous RNA (ceRNA) network.

Main Methods:

  • Utilized TCGA data (2006-2017) to identify differentially expressed pseudogenes, mRNAs, and microRNAs in GC.
  • Constructed a ceRNA network using miRWalk, miRTarBase, and DIANA-LncBase.
  • Validated in-silico findings using RT-qPCR on GC and adjacent normal tissue samples.

Main Results:

  • Identified 33 pseudogenes with significant expression changes in GC.
  • Pseudogenes RCN1P2, TPM3P9, and HSP90AB3P showed high connectivity within the ceRNA network.
  • Upregulated pseudogenes were associated with pathways in cell proliferation, inflammation, and metastasis, correlating with poor prognosis.

Conclusions:

  • Pseudogenes RCN1P2, TPM3P9, and HSP90AB3P are significantly upregulated in GC.
  • These pseudogenes potentially drive GC progression by modulating key cellular pathways via the ceRNA network, affecting patient survival.
Keywords:
Gene expressionMicroRNAsPseudogenesceRNAs network

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