Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of Deep Learning Approaches for Extreme Low-SNR Image Restoration.

GigaScience·2026

Same author

The US Public Health Service Commissioned Corps at the American Medical Association: A Model Cross-Sector Collaboration to Promote Better Health for All.

Public health reports (Washington, D.C. : 1974)·2026

Same author

Carboplatin, Cabazitaxel, and Abiraterone in High-Volume Metastatic Castration-Sensitive Prostate Cancer: The CASCARA Phase II Study.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

Same author

Correlative scanning electron and super-resolution structured illumination microscopy.

bioRxiv : the preprint server for biology·2026

Same author

Comparison of Deep Learning Approaches for Extreme Low-SNR Image Restoration.

bioRxiv : the preprint server for biology·2026

Same author

Low-Cost Spinning Disk Confocal Microscopy with a 25-Megapixel Camera.

Sensors (Basel, Switzerland)·2025

Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026

Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026

Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 12, 2025

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Spinning disk confocal microscopy with a 25 Megapixel Camera.

Guy M Hagen¹, Brian Lewis¹, Summer Levis¹

¹UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, Colorado, 80918, USA.

Biorxiv : the Preprint Server for Biology

|August 6, 2025

Summary

This summary is machine-generated.

This study introduces an affordable spinning disk confocal microscopy system using a CMOS camera. The low-cost setup provides high-resolution 3D imaging, making advanced microscopy more accessible.

More Related Videos

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

Related Experiment Videos

Last Updated: Sep 12, 2025

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

Published on: March 6, 2020

Area of Science:

Biomedical Imaging
Microscopy Technology
Optical Engineering

Background:

Spinning disk confocal microscopy offers fast optical sectioning and low phototoxicity.
High hardware costs limit accessibility of advanced spinning disk confocal microscopy systems.

Purpose of the Study:

To develop a low-cost spinning disk confocal microscopy system.
To demonstrate the feasibility of using affordable CMOS cameras in spinning disk microscopy.
To provide an open-source and scalable solution for advanced biological imaging.

Main Methods:

Utilized a 25-megapixel machine vision CMOS camera (Sony IMX540, FLIR Blackfly S).
Designed and constructed a custom spinning disk with 18 μm pinholes.
Integrated the camera and disk with an optical chopper motor and Micro-Manager software.

Main Results:

Achieved high-resolution 3D imaging of biological samples (mouse brain slices, rat testis).
Verified performance with high quantum efficiency (69% at 525 nm) and low read noise (2.31 electrons) CMOS sensor.
Demonstrated stable, near-telecentric imaging capabilities.

Conclusions:

Affordable CMOS sensors can effectively replace sCMOS cameras in spinning disk microscopy.
The developed system offers a cost-effective alternative for advanced imaging.
The open-source nature promotes scalability and wider adoption in research.