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High-Throughput Mechanical Characterization of Single Microgel Particles by Fluidic Force Microscopy.

Agnes Specht1, Steffen Trippmacher1, Nadine Raßmann1

  • 1Department of Physical Chemistry II, University of Bayreuth, Universitätsstraße 30, 95447, Bayreuth, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|August 8, 2025
PubMed
Summary

This study introduces an inverted nanoindentation technique using Fluidic Force Microscopy (FluidFM) for faster mechanical characterization of microgels. This method significantly speeds up the measurement of Young

Keywords:
atomic force microscopyfluidic force microscopymechanics of soft mattermicrogelsnanoindentation

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Area of Science:

  • Materials Science
  • Soft Matter Physics
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) based nanoindentation is standard for soft materials but time-consuming for individual microgel particles.
  • Accurate mechanical characterization of microgels requires measuring a large number of particles due to broad Young's moduli distribution.
  • Current methods face challenges in efficiently analyzing numerous micrometer-sized particles.

Purpose of the Study:

  • To develop a novel, high-throughput nanoindentation technique for soft particulate materials.
  • To reverse the roles of the indenter and sample in nanoindentation using Fluidic Force Microscopy (FluidFM).
  • To enable faster and more versatile mechanical characterization of microgels.

Main Methods:

  • Developed an 'inverted' nanoindentation approach utilizing Fluidic Force Microscopy (FluidFM).
  • Microgel particles are aspirated onto a FluidFM cantilever and indented against a flat substrate.
  • Experimental validation performed on polyoxazoline-hyaluronic acid (POx-HASH) and polyacrylamide (PAAm) microgels.

Main Results:

  • The inverted nanoindentation method achieves Young's moduli measurements 5-10 times faster than conventional AFM nanoindentation.
  • The technique successfully characterizes mechanical properties of diverse microgel types.
  • Measurements can be performed on various substrates, allowing investigation of adhesion effects.

Conclusions:

  • The inverted nanoindentation technique offers a significant advancement in the efficient mechanical characterization of microgel particles.
  • This method provides statistically reliable data more rapidly, crucial for understanding microgel behavior.
  • The versatility of the technique opens new avenues for studying soft matter interactions and substrate effects.