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QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

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Published on: June 22, 2017

Two distinct SWI/SNF complexes direct chromatin-linked transcriptional programs in Toxoplasma.

Dominic Schwarz^1,2, Benicio Tapia^1,2, Sebastian Lourido^1,2

¹Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.

Biorxiv : the Preprint Server for Biology

|August 12, 2025

Summary

Toxoplasma gondii

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Area of Science:

Molecular Biology
Genetics
Parasitology

Background:

Chromatin remodeling complexes regulate gene expression in eukaryotes.
Higher eukaryotes utilize diverse remodeler subtypes, but their coordination is unclear.
Apicomplexan parasites like Toxoplasma gondii offer a simpler model to study chromatin remodelers.

Purpose of the Study:

To comprehensively analyze the Myb protein family in Toxoplasma.
To define the composition and function of SWI3 complexes in this parasite.
To understand the distinct roles of BRG1 and BRM ATPases in chromatin regulation.

Main Methods:

Comprehensive analysis of the Myb protein family in Toxoplasma.
Definition of SWI3 complex composition using mutually exclusive ATPases (BRG1 and BRM).
Integration of transcriptomics with custom chromatin-profiling strategies.

Main Results:

Two distinct SWI3 complexes were identified, defined by BRG1 and BRM ATPases.
BRG1 is crucial for gene transcription during mitosis and cytokinesis.
BRM maintains global transcriptional competency and fidelity throughout the cell cycle.

Conclusions:

BRG1 and BRM exhibit distinct yet interdependent regulatory roles in Toxoplasma chromatin.
This study reveals ancestral principles of chromatin regulation and SWI/SNF complex diversification.
Findings offer insights into the functional specialization of chromatin remodelers across eukaryotes.