Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phases of Wound Repair

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization of Neuronal Ensembles in a Model of Dual-Reward Conditioned Place Preference.

eNeuro·2026

Same author

Bioinspired spatiotemporal control of microhelix formation and actuation.

Science advances·2026

Same author

Universal In Situ Flowrate Monitoring for Piezoelectric Microfluidics via Triboelectric Self-Sensing.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Synaptic plasticity in cocaine-seeking ensembles of the nucleus accumbens core.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026

Same author

Temporal Biomarker Profiles Following Non-Surgical Spinal Cord Concussion.

Journal of molecular neuroscience : MN·2026

Same author

Evaluation of median and obturator nerve fascicular topography using landmark morphometrics: a pilot study.

Folia morphologica·2026

Search research articles

Related Experiment Video

Updated: Sep 11, 2025

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

Targeting Cathepsin K to Accelerate Diabetic Wound Healing.

Anne Chenchar^1,2,3,4, Vitoria Mattos Pereira^1,2,3,4, Anisha Apte²

¹School of Pharmacy, Biomedical Sciences Graduate Program, Department of Zoology and Physiology, Department of Animal Sciences, University of Wyoming, 1000 E. University Avenue, Laramie, Wyoming 82071, United States.

ACS Pharmacology & Translational Science

|August 13, 2025

Summary

Inhibiting cathepsin K, a protease that impairs healing, significantly improved wound closure in diabetic pigs and mice. This suggests targeting cathepsin K could be a novel therapeutic strategy for chronic wounds.

Keywords:

cathepsin K diabetes mouse model odanacatib porcine model wound healing

More Related Videos

Protocol to Create Chronic Wounds in Diabetic Mice

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Published on: August 21, 2021

Related Experiment Videos

Last Updated: Sep 11, 2025

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Published on: February 17, 2023

Protocol to Create Chronic Wounds in Diabetic Mice

Protocol to Create Chronic Wounds in Diabetic Mice

Published on: September 25, 2019

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Published on: August 21, 2021

Area of Science:

Biochemistry
Dermatology
Pharmacology

Background:

Elevated protease activity, particularly cathepsin K, is linked to impaired wound healing.
Cathepsin K possesses collagenolytic and elastolytic properties that can degrade extracellular matrix components essential for repair.
Diabetic individuals often experience delayed wound healing due to various factors, including altered protease activity.

Purpose of the Study:

To investigate the therapeutic potential of inhibiting cathepsin K in diabetic wound healing.
To evaluate the efficacy of odanacatib, a cathepsin K inhibitor, in a diabetic porcine wound model.
To assess the impact of genetic cathepsin K deletion on wound healing in diabetic mice.

Main Methods:

Diabetic porcine and mouse models were established using streptozotocin (STZ).
Excisional skin wounds were created and treated with odanacatib or vehicle in pigs, and compared in cathepsin K knockout and wild-type mice.
Wound closure, epithelialization, CD31 expression, histology, and in vitro fibroblast/keratinocyte migration were assessed.

Main Results:

Odanacatib treatment significantly accelerated wound closure and improved epithelialization and ECM stability in diabetic pigs.
Diabetic mice lacking cathepsin K (Ctsk-/-) demonstrated enhanced wound healing compared to wild-type controls.
In vitro studies showed odanacatib promoted fibroblast and keratinocyte migration under high-glucose conditions.

Conclusions:

This study provides the first evidence that inhibiting cathepsin K, pharmacologically or genetically, enhances diabetic wound healing.
Cathepsin K represents a promising therapeutic target for managing chronic wounds, particularly in diabetic patients.