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RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
Published on: August 8, 2017
RGB Imaging and Irrigation Management Reveal Water Stress Thresholds in Three Urban Shrubs in Northern China
Yuan Niu1,2,3, Xiaotian Xu2,3, Wenxu Huang2,3,4
1Urban Forestry Research Center, Beijing Forestry University, Beijing 100083, China.
Abstract:
The context of global climate change, water stress has a significant impact on the ecological function and landscape value of urban greening shrubs. In this study, three typical greening shrubs (Euonymus japonicus, Ligustrum × vicaryi, and Berberis thunbergii var. atropurpurea) in North China were subjected to a two-year field-controlled experiment (2022-2023) with four water treatments: full irrigation, deficit irrigation, natural rainfall, and extreme drought. The key findings are as follows: (1) Extreme drought reduced the color indices substantially-the GCC of E. japonicus decreased by 40% (2023); the RCC of B. thunbergii var. atropurpurea declined by 35% (2022); and the color indices of L. × vicaryi remained stable (variation < 15%). (2) Early-season soil water content (SWC) strongly correlated with the color index of E. japonicus (r2 = 0.42, p < 0.05) but weakly with B. thunbergii (r2 = 0.28), suggesting species-specific drought-tolerance mechanisms like reduced leaf area. (3) Deficit irrigation (SWC ≈ 40%) maintained color indices between fully irrigated and drought-stressed levels. Notably, B. thunbergii retained high redness (RCC > 0.8) at an SWC ≈ 40%; E. japonicus required an SWC > 60% to preserve greenness (GCC). The research results provide a scientific basis for urban greening plant screening and water-saving irrigation strategies, and expand the application scenarios of color coordinates in plant physiological and ecological research.
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