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A highly sensitive fluorogenic assay for trypsin in urine: a simple diagnostic approach for pancreatic diseases
Jaemin Lim1, Hyeonjeong Seong1, Seoung Ho Lee1
1Department of Chemistry, Daegu University, Gyeongsan 38453, Republic of Korea. slee@daegu.ac.kr.
Organic & Biomolecular Chemistry
|August 18, 2025
Abstract:
A water-soluble pyrene-malonate (PyM) probe enables ultrasensitive, non-invasive detection of urinary trypsin for early pancreatic disease screening. Protamine binding induces aggregation and excimer formation, while trypsin-mediated cleavage disassembles the aggregates and enables monomer fluorescence recovery. The system reliably detects trypsin in 10% human urine within physiological levels.

