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Updated: Jan 13, 2026
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Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
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Tyrosinase detection via enzyme-triggered structural transformation of a ratiometric fluorescent probe
Minwoo Han1, Haemin Choi1, Juyeon Cha1
1Department of Chemistry, Daegu University, Gyeongsan 38453, Republic of Korea. slee@daegu.ac.kr.
Analytical Methods : Advancing Methods and Applications
|January 6, 2026
Abstract:
Tyrosinase is a key biomarker in melanoma, emphasizing the need for reliable detection. Here, we developed a ratiometric fluorescent probe that undergoes enzyme-triggered structural transformation, evidenced by HPLC and spectral changes. It enables sensitive analysis, inhibitor evaluation, and detection in spiked human serum, demonstrating potential for diagnostic applications.
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