Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Effect of DNase I on biofilm formation of Staphylococcus aureus].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2015

Same author

Dietary Fat Intake and Risk of Gastric Cancer: A Meta-Analysis of Observational Studies.

PloS one·2015

Same author

Overexpression of Gremlin promotes non-small cell lung cancer progression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine·2015

Same author

Nitrogen-Doped Porous Carbons As Electrode Materials for High-Performance Supercapacitor and Dye-Sensitized Solar Cell.

ACS applied materials & interfaces·2015

Same author

[Response and Control Factors of Groundwater to Extreme Weather, Jiguan Cave, Henan Province, China].

Huan jing ke xue= Huanjing kexue·2015

Same author

Quantum Storage of Three-Dimensional Orbital-Angular-Momentum Entanglement in a Crystal.

Physical review letters·2015

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 10, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Profiling Plant Nuclear Envelope Composition Using Subtractive Proteomics.

Xiao Liu¹, Yu Tang²

¹State Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences at Weifang, Weifang, Shandong, China.

Methods in Molecular Biology (Clifton, N.J.)

|August 20, 2025

Summary

This summary is machine-generated.

Researchers identified new nuclear envelope (NE) proteins in plants using a subtractive proteomic approach. This method advances understanding of plant cell nuclear envelope composition and function.

Keywords:

Endoplasmic reticulum Label-free quantification Mass spectrometry Nuclear envelope Plant nuclear envelope transmembrane proteins (PNETs)Subtractive proteomics YFP fusion

More Related Videos

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

Related Experiment Videos

Last Updated: Sep 10, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

Area of Science:

Plant Cell Biology
Proteomics
Molecular Biology

Background:

The nucleus, enclosed by the nuclear envelope (NE), is crucial for eukaryotic cells.
While NE composition is known in animals and yeast, it's less understood in plants.

Purpose of the Study:

To identify novel nuclear envelope components in the model plant Arabidopsis.
To establish a robust method for plant NE proteome profiling.

Main Methods:

Subtractive proteomics was employed.
Nuclei were isolated using Percoll density gradients.
Nuclear membranes were extracted, and proteomes determined.

Main Results:

Novel nuclear envelope proteins were profiled in Arabidopsis.
The method validated enrichment of proteins at the nuclear periphery.
The approach is adaptable for various plant tissues and species.

Conclusions:

This study provides a valuable method for plant nuclear envelope research.
It expands the understanding of plant cell biology and nuclear structure.