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Updated: Sep 10, 2025
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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
Published on: January 13, 2023
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How microsampling is impacting pharmacokinetic and toxicokinetic studies: volumetric absorptive microsampling (VAMS)
Michele Protti1, Roberto Mandrioli2, Hugo M Santos3,4,5
1Research group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Bologna, Italy.
Bioanalysis
|August 25, 2025
Summary
Microsampling, using tiny biological samples, offers advantages for pharmacokinetic and toxicokinetic studies. Volumetric absorptive microsampling (VAMS) is a key technology for reliable quantitative analysis in these studies.
Area of Science:
- Pharmacology and Toxicology
- Analytical Chemistry
- Biomedical Science
Background:
- Microsampling uses minimal biological specimens, offering advantages over traditional blood draws.
- Benefits include enhanced analyte stability, simplified logistics, and reduced material/animal consumption.
- Volumetric absorptive microsampling (VAMS) is a leading microsampling technique due to its volume accuracy.
Purpose of the Study:
- To review pharmacokinetic and toxicokinetic studies utilizing VAMS.
- To highlight the suitability of VAMS for quantitative analysis.
- To present analytical methods compatible with VAMS for these studies.
Main Methods:
- Literature review of VAMS applications in pharmacokinetic and toxicokinetic studies.
- Analysis of VAMS advantages over conventional sampling methods.
- Compilation of analytical techniques supporting VAMS implementation.
Main Results:
- VAMS demonstrates high performance and reliability comparable to traditional methods.
- VAMS offers significant improvements in sample stability and handling.
- Numerous analytical methods are available for VAMS-based quantitative analysis.
Conclusions:
- VAMS is a highly effective and advantageous microsampling technique for pharmacokinetic and toxicokinetic research.
- Its accuracy and the availability of analytical methods support its widespread adoption.
- VAMS represents a significant advancement in biological sample analysis for drug development and toxicology.
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