Related Experiment Video
Updated: Sep 10, 2025
Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
Published on: September 20, 2016
Preclinical Fluorescence-Guided Imaging Leveraging Surrounding Sentinel Tumor Microenvironment Identifies High-Risk
Shilpa Sharma1, Xiaoxia Wen1, Jianbo Wang1
1Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, Houston, Texas.
Purpose:
Because surgery is the only potential cure for pancreatic cancer, high-risk premalignant pancreatic lesions often evade detection by palpation or white-light visualization, increasing the risk of recurrence. We asked whether near-infrared fluorescence imaging of tumor-associated inflammation could identify high-risk premalignant lesions, leveraging the tumor microenvironment as a sentinel of local disease and, thus, enhance surgery outcomes.
Experimental Design:
Fluorescence-guided surgery was performed on genetically engineered mice [Ptf1a-Cre; LSL-KrasG12D/+; Smad4flox/flox (KSC)] at discrete stages of disease progression, histologically confirmed high-risk, premalignant lesions in postnatal mice to locally advanced pancreatic tumors in adults, using the imaging agent V-1520, a translocator protein ligand. Age-matched wild-type littermates were used as controls, whereas Ptf1a-Cre; LSL-KrasG12D/+ mice modeled pancreatitis and precursors of low penetrance. Localization of V-1520 and tumor-associated macrophages among the tumor microenvironment was detected by immunofluorescence imaging.
Results:
V-1520 exhibited robust accumulation in the pancreata of KSC mice from the early postnatal stage. Increased accumulation was observed in the pancreata of adolescent- and adult-aged mice with greater ductal lesion and stromal burden. Confocal microscopy of ex vivo pancreas specimens co-localized V-1520 accumulation primarily with CD68-expressing macrophages in KSC mice. Unlike the pancreata of KSC mice, accumulation of V-1520 did not exceed background levels in the pancreata of Ptf1a-Cre; LSL-KrasG12D/+ mice with pancreatitis.
Conclusions:
V-1520 exhibited differential accumulation in pancreatic cancer-associated inflammation compared with pancreatitis. Given the robust tracer uptake in tissues associated with early yet high-risk lesions, we envision that V-1520 could enhance surgical resection and reduce the potential for recurrence from residual disease.
