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Updated: Aug 11, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
[Amylase formation in a periodic and continuous culture of Bacillus subtilis]
Abstract:
The dynamics of alpha-amylase production by two strains of Bacillus subtilis (A32 and A32.6) was studied under periodic and continuous conditions of cultivation on a chemically defined medium and on a natural medium. In the periodic culture, the highest activity of the enzyme was found during the stationary growth phase. In the conditions of chemostat, as the dilution rate from 0.05 to 0.15 hr-1, the activity of amylase remained at the same, high level for a certain lapse of time and then decreased. The rate of the decrease of alpha-amylase activity depended on the dilution rate.
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