Evaluation of a new procedure for isoamylase measurement by selective inhibition

The authors have evaluated a new procedure for the estimation of pancreatic and salivary isoamylase using a new selective inhibitor reagent (Enzamyl Isoamylase, Gödecke), Germany; composition covered by patent No. P 3404876.6). Provided the conditions of the assay presented here are correctly selected, a standard curve is not necessary and the activities of both isoenzymes can be calculated by a simple formula. In view of its rapidity and precision, this new procedure appears to be useful as a screening test. It correlates closely with a reference electrophoretic method.