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Updated: Sep 9, 2025
06:28
Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
Published on: May 16, 2025
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The Endosperm-Specific Gene OsEnS-42 Regulates Seed Vigor and Grain Quality
Minhua Zheng1,2, Xiaodan Hu2, Luo Chen1,2
1College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Plants (Basel, Switzerland)
|August 28, 2025
Summary
The gene OsEnS-42 is crucial for rice seed vigor and grain quality. Knockout plants showed reduced vigor and poorer quality, but haplotype analysis offers breeding targets for improvement.
Area of Science:
- Plant genetics and breeding
- Molecular biology
- Agricultural science
Background:
- Seed vigor and grain quality are key determinants of rice yield and commercial value.
- Understanding the genetic basis of these traits is essential for crop improvement.
Purpose of the Study:
- To investigate the function of the endosperm-specific gene OsEnS-42 in rice seed development.
- To identify genetic targets for enhancing rice seed vigor and grain quality.
Main Methods:
- Gene knockout using CRISPR/Cas9 technology.
- Transcriptome analysis and physiological assays.
- Haplotype analysis across global rice germplasm.
Main Results:
- OsEnS-42 knockout decreased seed vigor (germination rate, seedling/root length) and increased grain chalkiness and amylose content.
- OsEnS-42 regulates seed vigor via soluble sugars and redox metabolism, and grain quality via soluble sugars and seed development enzymes.
- Haplotype analysis identified specific OsEnS-42 variants (Hap 4, Hap 2) associated with reduced chalkiness and lower amylose content, respectively.
Conclusions:
- OsEnS-42 plays a vital role in regulating rice seed vigor and grain quality.
- Specific OsEnS-42 haplotypes provide valuable targets for marker-assisted breeding to improve rice quality traits without subspecies limitations.
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