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Updated: May 1, 2026
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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
Published on: December 21, 2017
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Protocol for Disome-seq to identify transcriptome-wide ribosome collisions in yeast cells
Pedro H Ayres-Galhardo1, James Marks1, Sezen Meydan2
1Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
STAR Protocols
|August 28, 2025
Summary
Disomes, or ribosome collision complexes, form when translation elongation stalls. This new Disome-seq protocol allows researchers to detect disomes across the entire transcriptome in yeast cells.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Stalled ribosomes can lead to ribosome collisions, forming disomes.
- Disomes are cellular complexes that trigger signaling pathways.
- Understanding disome frequency and distribution is crucial for cellular regulation.
Purpose of the Study:
- To provide a detailed protocol for Disome-seq in Saccharomyces cerevisiae.
- To enable transcriptome-wide detection and analysis of disomes.
Main Methods:
- Yeast cell culture and growth.
- Isolation of ribosome collision complexes (disomes).
- Sequencing library preparation for transcriptome-wide analysis.
Main Results:
- The study presents a validated protocol for Disome-seq.
- This method allows for the detection of disomes across the yeast transcriptome.
Conclusions:
- The Disome-seq protocol facilitates the study of ribosome collisions in yeast.
- This technique is essential for investigating the biological roles and consequences of disome formation.
