Enhanced chemiluminescence driven by Fe-MIL-101(NH₂) host-guest recognition for the detection of glutamic acid

Enhancing luminol-based chemiluminescence (CL) emission through functionalized materials is essential for developing highly sensitive method in rapid analysis. Herein, a Fe-MIL-101(NH₂) enhanced CL method was developed for the selective determination of glutamic acid (Glu) in serum. By utilizing Fe-MIL-101(NH₂) as an effective catalyst for the first time, the CL intensity of the luminol-H₂O₂ system was amplified, and this system exhibited a selective response to Glu. Mechanism studies demonstrated that Fe-MIL-101(NH₂) selectively recognizes HOO^- and Glu through host-guest interactions, thereby accelerating the luminol-H₂O₂ reaction kinetics. Under optimal conditions, the CL intensity exhibited a linear relationship with the concentration of Glu in the range of 0.01-10 μg/mL, with the limited of detection (LOD) of 0.003 μg/mL. This method was successfully applied to determine Glu in human serum from epilepsy patients and healthy individuals, with results that consistent with those from high-performance liquid chromatography-mass spectrometry (HPLC-MS). New applications were opened up for CL and provided a rapid and reliable alternative method for diagnosing mental disorders.