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Updated: Sep 9, 2025

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
Published on: April 4, 2019
Minigenome System to Study Respiratory Syncytial Virus Transcription Versus Genome Replication
1Department of Virology, Immunology & Microbiology, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
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The respiratory syncytial virus (RSV) minigenome system is a powerful analytical tool that has enabled viral transcription and genome replication to be modeled in a cellular context without viral infection. The minigenome assay can be used to dissect the different steps in RSV transcription and genome replication, assess the effects of mutations in cis-acting RNA elements, analyze mutations in the polymerase and other proteins in the transcription-replication machinery, and assess the effect of antiviral compounds on RSV polymerase functions. Here, we describe the RSV minigenome assay and detail a northern blotting technique for detecting viral mRNA transcripts and genome replication products.
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