Analysis of Serum Serotonin by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using Online...

Area of Science:

• Clinical Chemistry
• Analytical Chemistry
• Biochemistry

Background:

• Serotonin plays a crucial role in various physiological processes.
• Accurate quantification of serotonin in blood serum is essential for diagnosing and managing associated diseases.
• Existing methods may face challenges with matrix effects and sample preparation efficiency.

Purpose of the Study:

• To develop and validate a robust method for the quantitative determination of serotonin in human blood serum.
• To establish a reliable analytical technique for assessing serotonin levels in clinical settings.
• To support the diagnosis and monitoring of conditions linked to altered serotonin metabolism.

Main Methods:

• High-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS).
• Online solid-phase extraction integrated within the chromatographic system for automated sample preparation.
• Validation of the method for accuracy, precision, linearity, and sensitivity.

Main Results:

• The developed method enables accurate serotonin quantification in blood serum over a wide linear range (0.5-2000 ng/ml).
• Online solid-phase extraction effectively minimizes matrix component interference, enhancing assay reliability.
• The method demonstrates high sensitivity and specificity for serotonin determination.

Conclusions:

• The proposed HPLC-MS/MS method offers a reliable and efficient approach for serotonin measurement in blood serum.
• This technique facilitates the quantitative assessment of serotonin levels, crucial for clinical diagnostics.
• The method's automation and reduced matrix effects make it suitable for routine clinical laboratory use and research on serotonin-associated pathologies.