Analysis of Serum Serotonin by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using Online
D V Bychenkov1, V A Ioutsi2, I V Kirilina2
1National Medical Research Center for Endocrinology, Ministry of Health of the Russian Federation, Moscow, Russia. bychenkov.denis@endocrincentr.ru.
Abstract:
We propose a method for the quantitative determination of serotonin in blood serum using high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and online solid-phase extraction in a chromatographic system. This approach allows serotonin quantification in blood serum within a range of 0.5-2000 ng/ml, partially automates the sample preparation process, and minimizes the influence of matrix components. The method is designed for the quantitative measurement of serotonin levels in patients with various deviations of serotonin levels and associated diseases.
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